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1. 发明授权

US5200311A Method of determination of pesticides by radiobiochemistry 失效
标题翻译：通过放射生物化学测定农药的方法
公开(公告)号：US5200311A
公开(公告)日：1993-04-06
申请号：US556952
申请日：1990-07-19
申请人： Stanley E. Charm , Shlomo Capua , Eliezer Zomer
发明人： Stanley E. Charm , Shlomo Capua , Eliezer Zomer
IPC分类号： G01N33/567 , G01N33/60
CPC分类号： G01N33/567 , G01N33/60 , Y10S436/804
摘要： A biochemical system in the determination of pesticides which is able to detect and measure the presence of parts per billion levels of different pesticides particularly in foodstuffs. The method employs insect tissues that contain specific binders or enzymes sensitive to various groups of pesticides. The sample or sample extract containing pesticides, radiolabeled pesticides and/or radiolabeled pesticide analogs and/or radiolabeled substrates is exposed to insect tissues containing receptors or enzymes. The pesticide competes with the radioactive tracers for the binders or the enzymes in the insect tissues. Residual enzyme activity or bound pesticides are measured. The extent of binding or enzyme activity may be inversely proportional, under standardized conditions, to the amount of pesticide in the sample.
摘要翻译：一种生化系统，用于确定农药，能够检测和测量特别是在食品中存在十亿分之几的不同农药。该方法使用含有对各种农药敏感的特异性结合剂或酶的昆虫组织。含有农药，放射性标记的农药和/或放射性标记的农药类似物和/或放射性标记的底物的样品或样品提取物暴露于含有受体或酶的昆虫组织。杀虫剂与放射性示踪剂竞争昆虫组织中的结合物或酶。测量残留的酶活性或结合的农药。在标准条件下，结合或酶活性的程度可能与样品中农药的量成反比。

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